What is the advantage of incoporation of Unique Molecular Identifiers (UMI)?

Incorporated Unique Molecular Identifiers (UMI) eliminate PCR and sequencing errors, reduce the number of duplicates and ensure a greater confidence in clonotype identification, allowing a correct clonotype call at a lower sequencing depth, than non UMI pipeline.Immune profiling [...]

What software should I use for the analysis of the TCR a/b sequencing reads generated using the SMARTer Human TCR a/b Profiling Kit v2?

For the analysis of the TCR a/b sequencing reads generated using the SMARTer Human TCR a/b Profiling Kit v2 we recommend free-of charge Cogent NGS Immune Profiler Software.Immune profiling [...]

Is the SMARTer Human TCR a/b Profiling Kit v2 compatible with whole transcriptome profiing?

No - SMARTer Human TCR a/b Profiling Kit v2 has been designed and validated for TCR-specific amplification only.Immune profiling [...]

What cells are compatible with the SMARTer® Human TCR a/b Profiling Kit?

SMARTer® Human TCR a/b Profiling Kit has been validated with 1,000 - 10,000 purified whole intact T cells, ensuring efficient dT priming of intact polyA RNA. Damaged cells are not suitable for the SMARTer® Human TCR a/b Profiling Kit, since such cells contain compromised RNA.Immune profiling [...]

What is the recommended sequencing depth for TCR profiling.

The recommended sequencing depth for TCR profiling depends on the input RNA/ cell quantity defining the sample complexity and is provided in the SMARTer Human TCR a/b Profiling Kit v2 User Manual - Table 6. Recommended sequencing depth for TCR libraries prepared from human PBMC RNA, human whole blood RNA, or T-cell RNAImmune profiling [...]