Can I use indexing primers from vendors other than Takara Bio USA?

Indexing primers from vendors other than Takara Bio USA, are not compatible with the SMARTer® smRNA-Seq Kit for Illumina, since within the workflow of the SMARTer® smRNA-Seq Kit for Illumina, PCR incorporation of indexing primers requires proprietary overlaps with the first-strand cDNA template as shown in the SMARTer® smRNA-Seq Kit for Illumina User Manual - Figure 1. Schematic of technology [...]

Can I perform SMARTer smRNA-Seq library size selection using polyacrylamide gel?

SMARTer smRNA-Seq Kit workflow has not been validated with polyacrylamide gel, but only with BluePippin and AMPure beads, as described in the SMARTer smRNA-Seq Kit for Illumina User ManualsmRNA-Seq [...]

What is the origin of prominent peaks in the electropherogram of the SMARTer smRNA library generated from total RNA?

The prominent peaks in the SMARTer smRNA cDNA traces represent abundant RNAs: for example, 5S rRNA is about 120nt plus 153bp primer-dimer – would constitute about 300bp – see the corresponding 300bp peak in Fig.3C of the SMARTer smRNA-seq Kit for Illumina User Manual.smRNA-Seq [...]

Is SMARTer smRNA-Seq Kit for Illumina compatible with bacterial RNA?

Theoretically, SMARTer® smRNA-Seq Kit for Illumina should be compatible with bacterial RNA, though we have not validated this kit with bacterial RNA. However, if bacterial small RNAs have strong secondary structures, it may present a challenge.smRNA-Seq products Care guide Caution [body] This care sheet provides general information only for handling Carolina™ bacterial cultures. When you work [...]

Will Poly(A) Polymerase add As to the DNA oligo carrier present in the RNA prep?

Poly(A) Polymerase does not add As to ssDNA.smRNA-Seq [...]