What is the origin of prominent peaks in the electropherogram of the SMARTer smRNA library generated from total RNA?
What is the origin of prominent peaks in the electropherogram of the SMARTer smRNA library generated from total RNA?
The prominent peaks in the SMARTer smRNA cDNA traces represent abundant RNAs: for example, 5S rRNA is about 120nt plus 153bp primer-dimer – would constitute about 300bp – see the corresponding 300bp peak in Fig.3C of the SMARTer smRNA-seq Kit for Illumina User Manual.