Can I use purification beads from vendor/ source other than recommended in the SMART-Seq® mRNAKit for Sequencing User Manual.

No, please, use only the magnetics beads recommended in the SMART-Seq® mRNA Kit for Sequencing User Manual - III. Additional Materials Required -For Bead Purifications. Since SeqAmp PCR reaction occurs in the pressence of beads it is essential to use only the beads validated with the SMART-Seq® mRNA Kit [...]

Can I use 5X First-Strand Buffer supplied with the SMARTScribe™ Reverse Transcriptase as a substitute for the 5X Ultra Low First-Strand Buffer supplied with theSMART-Seq® mRNA Kit ?

No, 5X Ultra Low First-Strand Buffer supplied with the SMART-Seq® mRNAKit includes also DTT and dNTPs, that are not included n the 5X First-Strand Buffer supplied with the SMARTScribe™ Reverse Transcriptase.Ultra-low input NGS-RNA [...]

How many reactions can I perform using different size formats of the SMART-Seq® mRNA Kit ?

Each kit size indicates the number of total reactions accommodated by the kit, including Positive and Negative controls. Performing Negative and positive controls for each set of the experiments is essential: Positive Control using Control Total RNA ensures the performance of kit components; No RNA/Negative Control ensures the absence of environmental contaminationUltra-low input NGS-RNA [...]

Why do I need to perform Positive and Negative controls?

Performing Negative and positive controls for each set of the experiments is essential: Positive Control using Control Total RNA ensures the performance of kit components; No RNA/Negative Control ensures the absence of environmental contamination Ultra-low input NGS-RNA [...]

What is the difference between SMART-Seq2 and SMART-Seq® mRNA kit?

SMART-Seq2 is a lab-based protocol utilizing Takara Bio USA SMART(er) cDNA synthesis technology. SMART-Seq® mRNA Kit, shows a superior performance over the SMART-Seq2, ensuring a better representation of rare transcripts, higher mapping and reproducibility rates as described in the Technical Note -Improving the sensitivity of ultra-low input mRNA-seq.Ultra-low input NGS-RNA [...]