Can I use purification beads from vendor/ source other than recommended in the SMART-Seq® mRNAKit for Sequencing User Manual.
Can I use purification beads from vendor/ source other than recommended in the SMART-Seq® mRNAKit for Sequencing User Manual.
No, please, use only the magnetics beads recommended in the SMART-Seq® mRNA Kit for Sequencing User Manual - III. Additional Materials Required -For Bead Purifications. Since SeqAmp PCR reaction occurs in the pressence of beads it is essential to use only the beads validated with the SMART-Seq® mRNA Kit workflow.