Can cells collected in QIAGEN Buffer RLT used as input for the SMART-Seq® mRNA cDNA synthesis?

QIAGEN Buffer RLT or a similar buffer, containing guanidine thiocyanate - a protein denaturant - is not compatible with the SMART-Seq mRNA cDNA synthesis. Cells collected in Buffer RLT, have to be used for RNA isolation/ purification prior to cDNA synthesis Ultra-low input NGS-RNA [...]

Can RNA or cells stored in RNAlater be used as input for the SMART-Seq® mRNA cDNA synthesis?

RNAlater is NOT compatible with the SMART-Seq® mRNA cDNA synthesis, since it denatures proteins. RNA from cells stored in RNAlater must be purified prior to use as input for the SMART-Seq® Ultra® Low cDNA synthesis.Ultra-low input NGS-RNA [...]

Can I use a carrier or co-precipitant during purification of RNA intended for the SMART-Seq mRNA cDNA synthesis?

It is advisable not to use any carrier or co-precipitant during RNA purification since it may distort RNA concentration and interfere with cDNA synthesisUltra-low input NGS-RNA [...]

What can I do when input RNA exhibiting a low 260/230 OD ratio?

A low 260/230 OD ratio may indicate the presence of organic compounds in the RNA prep which may interfere with the SSMART-Seq mRNA cDNA synthesis. To ensure the absence of organic compounds in the RNA prep intended for the SMART cDNA synthesis perform additional purification of your RNA using NucleoSpin® RNA Clean-up XS - [...]

What is the source of SMART-Seq® mRNA Kit control RNA ?

SMART-Seq® mRNA kit includes Control Mouse Brain Total [...]