What can I do when input RNA exhibiting a low 260/230 OD ratio?
What can I do when input RNA exhibiting a low 260/230 OD ratio?
A low 260/230 OD ratio may indicate the presence of organic compounds in the RNA prep which may interfere with the SSMART-Seq mRNA cDNA synthesis. To ensure the absence of organic compounds in the RNA prep intended for the SMART cDNA synthesis perform additional purification of your RNA using NucleoSpin® RNA Clean-up XS -
http://www.clontech.com/US/Products/Nucleic_Acid_Purification/RNA/RNA_Clean-Up?sitex=10020...