Does the PicoPLEX Gold Single Cell DNA-Seq Kit's coverage span GC-rich regions?

More than 90% of the reads from libraries generated with the PicoPLEX Gold Single Cell DNA-Seq Kit fall between 25% and 70% GC content.DNA-seq [...]

What ratio of AMPure XP beads should I use for purifying the library prepared with the PicoPLEX Gold Single Cell DNA-Seq Kit?

Mix the beads and the sample(s) at a 1:1 ratio for most NGS-based CNV detection purposes.DNA-seq [...]

What concentration of PicoPLEX Gold Single Cell DNA-Seq library should be loaded onto the Illumina MiSeq flow cell?

With libraries made from a single cell using the PicoPLEX Gold Single Cell DNA-Seq Kit, a good starting point is to consider a library size of 300 bp and load 16 pM on the MiSeq® v3. Other platforms will have different loading concentrations, so we recommend following the manufacturers' instructions. It is very important to add at least 5% PhiX DNA to the library prior to loading on the flow [...]

How do I quantify the pooled, purified library prepared by PicoPLEX Gold Single Cell DNA-Seq Kit for loading onto an Illumina sequencer?

Quantify PicoPLEX Gold Single Cell DNA-Seq libraries using real-time qPCR using the appropriate instrument-specific Illumina library quantification kit. Pooled, purified libraries are diluted 50,000–500,000-fold and used as the template for quantification by real-time qPCR. It is recommended to use 300 bp as the size for calculating the library concentration.DNA-seq [...]

What is the recommended fluorescent dye to monitor amplification using a real-time PCR cycler?

EvaGreen dye (Cat. No. 31000-T, Biotium Inc.) is recommended due to its high sensitivity and low interference with amplification chemistry. Depending on the real-time instrument used, a calibration dye may also be needed. Please refer to your real-time PCR instrument's user manual for additional information. DNA-seq [...]