How do I quantify the pooled, purified library prepared by PicoPLEX Gold Single Cell DNA-Seq Kit for loading onto an Illumina sequencer?
How do I quantify the pooled, purified library prepared by PicoPLEX Gold Single Cell DNA-Seq Kit for loading onto an Illumina sequencer?
Quantify PicoPLEX Gold Single Cell DNA-Seq libraries using real-time qPCR using the appropriate instrument-specific Illumina library quantification kit. Pooled, purified libraries are diluted 50,000–500,000-fold and used as the template for quantification by real-time qPCR. It is recommended to use 300 bp as the size for calculating the library concentration.