Why should partial media changes be performed from Day 3 onward?

Repeated drying, which easily happens when changing 100% of the media, detaches the cells at the edges of the well. Changing only 90% of the media ensures that liquid is always left on the cells, preventing desiccation and detachment. For washing steps in assays or immunocytochemical staining, it is better to leave a small amount of buffer or washing solution on the cells and increase the number [...]

Why do the cells need to be washed on Day 1 after plating?

Washing with Hepatocyte Washing Medium on Day 1 after plating is necessary to remove nonadherent cells. When washing, carefully remove 100% of the media from only a few wells at a time. Do not let the wells dry. Stem Cell [...]

Does Cellartis Enhanced hiPS-HEP Long-Term Maintenance Medium contain dexamethasone, hydrocortisone, and DMSO?

Yes, Cellartis Enhanced hiPS-HEP Long-Term Maintenance Medium contains all three of these components. We have not evaluated the performance of Cellartis Enhanced hiPS-HEP cells in the absence of dexamethasone, hydrocortisone, or DMSO. Stem Cell [...]

Can other maintenance media or coatings be used for culturing Cellartis enhanced hiPS-HEP cells?

We recommend using the provided Cellartis Enhanced hiPS-HEP Thawing and Plating Kit and Cellartis Enhanced hiPS-HEP Long-Term Maintenance Medium. These components are included within the Cellartis enhanced hiPS-HEP v2 kits, and they are crucial for optimal performance and results with the included Cellartis enhanced hiPS-HEP cells. Other coatings or media can be used, however, we cannot [...]

Which culture vessels are suitable for Cellartis enhanced hiPS-HEP cells?

96-well and 24-well plates are recommended.Stem Cell [...]