Why should partial media changes be performed from Day 3 onward?
Why should partial media changes be performed from Day 3 onward?
Repeated drying, which easily happens when changing 100% of the media, detaches the cells at the edges of the well. Changing only 90% of the media ensures that liquid is always left on the cells, preventing desiccation and detachment. For washing steps in assays or immunocytochemical staining, it is better to leave a small amount of buffer or washing solution on the cells and increase the number of washing steps.