How are Cellartis enhanced hiPS-HEP cells shipped?

Cellartis enhanced hiPS-HEP cells are frozen in a cryovial and packaged as a part of a Cellartis enhanced hiPS-HEP v2 kit. This kit also contains the Cellartis Enhanced hiPS-HEP Thawing and Plating Kit and Cellartis Enhanced hiPS-HEP Long-Term Maintenance Medium. All items are shipped together on dry ice. Stem Cell [...]

Do Cellartis enhanced hiPS-HEP cells show formation of bile canaliculi?

We have not been able to show the formation of bile canaliculi in these cellsStem Cell [...]

How do we know Cellartis enhanced hiPS-HEP cells are mature hepatocytes?

To determine the maturity of the cells, we investigated genes known to be expressed in fetal and adult hepatocytes and tested by multiple assays how they compare to primary hepatocytes. We observed high expression and activity of multiple adult hepatocyte markers such as albumin, CYP1A2, CYP2C9, CYP2C19, CYP3A4, HNF?, and ASGPR1. We confirmed this by immunofluorescence, activity, and qPCR [...]

Cellartis enhanced hiPS-HEP cells are >90% pure. Which cell types make up the other 10%?

This subpopulation is likely a mixture of other endodermal cells, but the exact composition is unknown. We start with high-purity endodermal cells in the first step of differentiation, and we confirm through staining that there are no undifferentiated stem cells in the final product. Stem Cell [...]

Does fetal CYP3A7 contribute to overall CYP activity in these cells?

Whereas a CYP activity assay cannot discriminate between CYP3A4 (adult), CYP3A5 (adult), and CYP3A7 (fetal), qPCR can. In our tests, Cellartis enhanced hiPS-HEP cells expressed high mRNA levels of CYP3A4 and CYP3A5 and very low levels of CYP3A7—comparable to cryopreserved human primary hepatocytes cultured for 24 hours (Panel A). Interestingly, interindividual variation in CYP expression can be [...]