How do we know Cellartis enhanced hiPS-HEP cells are mature hepatocytes?
How do we know Cellartis enhanced hiPS-HEP cells are mature hepatocytes?
To determine the maturity of the cells, we investigated genes known to be expressed in fetal and adult hepatocytes and tested by multiple assays how they compare to primary hepatocytes. We observed high expression and activity of multiple adult hepatocyte markers such as albumin, CYP1A2, CYP2C9, CYP2C19, CYP3A4, HNF?, and ASGPR1. We confirmed this by immunofluorescence, activity, and qPCR analyses. We also observed a low expression level of the fetal gene CYP3A7—similar to the level in primary hepatocytes.