Can the PicoPLEX Gold Single Cell DNA-Seq Kit be used without a real-time PCR machine?

A real-time PCR cycler is recommended to monitor amplification by the addition of fluorescent dye (not provided with the kit) to the reaction. If a regular thermal cycler is used instead, there is no need to add the dye. Substitute with an appropriate amount of nuclease-free water to adjust volume while preparing the Amplification Reaction Master Mix. In the absence of a real-time thermal [...]

Can the PicoPLEX Gold Single Cell DNA-Seq Kit be run on any thermal cycler?

The PicoPLEX Gold Single Cell DNA-Seq Kit must be run on a thermal cycler that can accommodate 50-µl reaction volumes and that is equipped with a heated lid. Set the temperature of the heated lid to 100–105°C to avoid sample evaporation during incubation and cycling.DNA-seq [...]

What is the size range of fragments expected with libraries prepared using the PicoPLEX Gold Single Cell DNA-Seq Kit?

Libraries prepared using the PicoPLEX Gold Single Cell DNA-Seq Kit result in a broad size-range distribution of fragments, typically ranging from ~300–1,000 bp total size (~200–900 bp insert size).DNA-seq [...]

Can I prepare samples for both single- and paired-end NGS sequencing using Takara PicoPLEX Gold Single Cell DNA-Seq kits?

Libraries prepared using the PicoPLEX Gold Single Cell DNA-Seq Kit are compatible with both single- and paired-end sequencing on Illumina platforms.DNA-seq [...]

Can the PicoPLEX Gold Single Cell DNA-Seq Kit be used with Ion Torrent or Pac Bio platforms?

No, this kit is not compatible with Ion Torrent or Pac Bio.DNA-seq [...]