Can the PicoPLEX Gold Single Cell DNA-Seq Kit be used without a real-time PCR machine?
Can the PicoPLEX Gold Single Cell DNA-Seq Kit be used without a real-time PCR machine?
A real-time PCR cycler is recommended to monitor amplification by the addition of fluorescent dye (not provided with the kit) to the reaction. If a regular thermal cycler is used instead, there is no need to add the dye. Substitute with an appropriate amount of nuclease-free water to adjust volume while preparing the Amplification Reaction Master Mix. In the absence of a real-time thermal cycler, use a regular thermal cycler and quantify library amplification via gel or Bioanalyzer analysis.