How many bases should I trim for analysis of the sequencing reads from PicoPLEX Gold Single Cell DNA-Seq Kit?

The first 11 cycles of each read will contain quasi-random bases introduced during the PicoPLEX Gold Single Cell DNA-Seq library preparation. For sequence alignment, either trim the initial 14 bases from each read or begin calibration and data collection at base position 15.DNA-seq [...]

What are the indexing options for the PicoPLEX Gold Single Cell DNA-Seq Kit?

The DNA single index, unique dual index, and HT dual index kits are compatible with the PicoPLEX Gold Single Cell DNA-Seq Kit.DNA-seq [...]

Can we use barcodes designed in our laboratory for PicoPLEX Gold Single Cell DNA-Seq Kit?

No. Only DNA single index, unique dual index, and HT dual index kits have been approved for use with the PicoPLEX Gold Single Cell DNA-Seq Kit.DNA-seq [...]

Do we have to purchase barcoded oligonucleotides separately for PicoPLEX Gold Single Cell DNA-Seq Kit?

Yes. Our DNA single index, unique dual index, and HT dual index kits are compatible with the PicoPLEX Gold Single Cell DNA-Seq Kit.DNA-seq [...]

Can cells stained with surface antibodies be used as input for PicoPLEX Gold Single Cell DNA-Seq Kit?

Yes, cells stained with surface antibodies can be used if the cells are not fixed.DNA-seq [...]