How many bases should I trim for analysis of the sequencing reads from PicoPLEX Gold Single Cell DNA-Seq Kit?
How many bases should I trim for analysis of the sequencing reads from PicoPLEX Gold Single Cell DNA-Seq Kit?
The first 11 cycles of each read will contain quasi-random bases introduced during the PicoPLEX Gold Single Cell DNA-Seq library preparation. For sequence alignment, either trim the initial 14 bases from each read or begin calibration and data collection at base position 15.