What cell types have been successfully used with the PicoPLEX Gold Single Cell DNA-Seq Kit?

Single blastomeres, trophectoderm cells, and cultured cells have been used successfully.DNA-seq [...]

How rapid is the PicoPLEX Gold Single Cell DNA-Seq Kit?

The PicoPLEX Gold Single Cell DNA-Seq Kit lyses cells and prepares appropriately-barcoded NGS-ready libraries in less than 3 hours.DNA-seq [...]

Can I use the PicoPLEX Gold Single Cell DNA-Seq Kit for whole-genome de novo sequencing from a single cell?

No. The PicoPLEX Gold Single Cell DNA-Seq Kit offers robust and reproducible amplification of DNA from single cells for the detection of copy number variations (CNVs), single nucleotide variants (SNVs), and other chromosomal anomalies. This kit should not be used for high-coverage deep sequencing such as de novo sequencing and/or complete (whole genome) resequencing.DNA-seq [...]

What applications are recommended for the PicoPLEX Gold Single Cell DNA-Seq Kit?

The PicoPLEX Gold Single Cell DNA-Seq Kit is used to amplify genomic DNA from single cells to reliably detect chromosomal aneuploidies, copy number variations (CNVs), and single nucleotide variants (CNVs) using next-generation sequencing on Illumina platforms. Applications include preimplantation genetic testing (PGT) using blastomeres and trophectoderm cells as well as analysis of chromosomal [...]

Do PicoPLEX Gold Single Cell DNA-Seq Kit use single or dual indexes?

There are a variety of different indexing options available for use with our DNA-seq kits. Choose from the following single and dual index kits: 1. DNA HT Dual Index kits DNA HT Dual Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the [...]