Do PicoPLEX Gold Single Cell DNA-Seq Kit use single or dual indexes?
Do PicoPLEX Gold Single Cell DNA-Seq Kit use single or dual indexes?
There are a variety of different indexing options available for use with our DNA-seq kits. Choose from the following single and dual index kits:
1. DNA HT Dual Index kits
DNA HT Dual Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the Illumina Nextera® XT v2 index sequences and offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in four different barcoded index plates, each of which contains one-fourth of the possible dual index combinations (Cat. Nos. R400660–R400663). They are alternately available in a set of 24 individual tubes, each containing a different dual index combination (Cat. No. R400664). Each dual index tube (24N) contains sufficient volume for up to two uses. Each well of a dual index plate (96N Sets A–D) contains sufficient volume for a single use.
2. DNA Unique Dual Index kits
DNA Unique Dual Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the ""IDT for Illumina UD"" index sequences and offer a total of 96 dual indexes for multiplexing up to 96 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 24 individual tubes, each of which contains one-fourth of the possible dual index combinations (Cat. Nos. R400665–R400668). Each dual index tube (24U Sets A–D) contains sufficient volume for up to two uses.
3. DNA Single Index kits
DNA Single Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the ""TruSeq LT set A"" index sequences and offer a total of 12 single indexes for multiplexing of up to 12 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 12 individual tubes, each containing a different index sequence (Cat. Nos. R400695 and R400697). Each single index tube (12S Sets A–B) contains sufficient volume for up to eight uses.