What is the size range of CNVs that can be detected using the PicoPLEX Gold kit?

We showed that we can detect CNV of different sizes depending on the depth of sequencing. For example, 10 Mega bases CNV can be detected from 500,000 reads. Smaller CNV (as few hundred kilobases (100 kb)) can be detected with deeper sequencing. The Figure 5, of our PicoPLEX Gold app note, showed CNV detection of 100kb. [...]

Are the PicoPLEX WGA and GOLD kits compatible with the higher number of cells - > 100 cells?

We have successfully tested the PicoPLEX GOLD with 100 cells (~600 pg), but the goal was to make it work with 1-5 cells. So, all reaction optimizations were done to amplify a single cell genome. So, higher than recommended inputs are possible for Picoplex kits, but excessive use of template will defeat the purpose of a low-input chemistry. We have not tested the PicoPLEX kits with 1ng of [...]

Can I use the PicoPLEX WGA kit downstream of the RT (reverse transcription) reaction to analyze clinical samples infected by various pathogens – DNA and RNA based viruses and bacteria?

We have never utilized the PicoPLEX kits downstream of RT reaction. However, there is an interesting article you may want to check - https://www.frontiersin.org/articles/10.3389/fcimb.2018.00375/full Front. Cell. Infect. Microbiol., 23 October 2018 | https://doi.org/10.3389/fcimb.2018.00375 Authors diluted the RT reaction to 50 pg/?l of which, 1 ?l diluted RT product was mixed with 4 ?l of Cell [...]

Do all three generations of PicoPLEX kits have the same Extraction buffer?

Yes. The Extraction buffers are same in all PicoPLEX kits. DNA-seq [...]

Are the PicoPLEX Gold libraries compatible with the Illumina Patterned Flow cell sequencing platforms such as Novaseq 6000?

Yes, you can use the commercially available Target Enrichment probes with the PicoPLEX Libraries. However, you should keep in mind that PicoPLEX libraries have a larger than average size – 800 to 1000 bp. The commercial Enrichment probes are much shorter than PicoPLEX libraries. So, the pulled down region will be more than the bait region. This will cause somewhat reduced absolute % on-target. [...]