What are the storage conditions for your DNA-seq kits?

In general, our DNA-seq kits should be stored at –20°C. Consult your product's Certificate of Analysis for specific storage guidelines.DNA-seq [...]

Can I use indexing primers from vendors other than Takara Bio USA?

Indexing primers from vendors other than Takara Bio USA, are not compatible with the DNA SMART™ ChIP-Seq Kit, since within the workflow of the DNA SMART™ ChIP-Seq Kit, PCR incorporation of indexing primers requires proprietary overlaps with the first-strand cDNA template as shown in the DNA SMART™ ChIP-Seq Kit User Manual - Figure 1. Flowchart of technology in the DNA SMART ChIP-Seq [...]

What is the purpose of the DNA SMART Custom Read2 Seq Primer, include in the DNA SMART™ ChIP-Seq Kit?

if paired-end sequencing is desired, we recommend using the DNA SMART Custom Read2 Seq Primer provided in the kit, that will allow sequencing to start right after the priming site of the DNA SMART Poly(dA) Primer, thus avoiding reading through the A/T tail created on the 3’ end of ssDNA molecules. Sequencing through the stretch of adenine bases may result in low sequencing performance due to [...]

What is the reproducibility of the DNA SMART ChIP-seq libraries?

Libraries generated using DNA SMART ChIP-seq kit have high reproducibility: the overlap between the number of peaks identified from technical replicates generated from the same amount of input DNA is >93% for input levels greater than 100 pg, and libraries generated from different input amounts have >94% overlap with each other, as shown in the Technical Note Ligation-free ChIP-seq library [...]

Can the DNA SMART Poly(dA) Primer  anneal to internal /A -rich sequences in the genomic DNA?

DNA SMART Poly(dA) Primer can anneal to the T/A -rich sequences in the genomic DNA. The level of T/A noise in the final DNA SMART™ ChIP library may vary between different target DNAs. To remove the background noise consider a software tool described by Zhao and Zheng BMC Bioinformatics (2018) 19:544 - SMARTcleaner: identify and clean off-target signals in SMART ChIP-seq analysisChIP-seq and [...]