What is the purpose of the DNA SMART Custom Read2 Seq Primer, include in the DNA SMART™ ChIP-Seq Kit?
What is the purpose of the DNA SMART Custom Read2 Seq Primer, include in the DNA SMART™ ChIP-Seq Kit?
if paired-end sequencing is desired, we recommend using the DNA SMART Custom Read2 Seq Primer provided in the kit, that will allow sequencing to start right after the priming site of the DNA SMART Poly(dA) Primer, thus avoiding reading through the A/T tail created on the 3’ end of ssDNA molecules. Sequencing through the stretch of adenine bases may result in low sequencing performance due to low complexity