Can I validate the size distribution of the DNA SMART ChIP libraries by garose gel?

Agarose gel lacks the sensitivity for the validation of the DNA SMART ChIP libraries. Agarose gel lacks the sensitivity for the validation of the DNA SMART ChIP libraries. To minimize biases, ChIP-seq libraries are amplified at a minimal optimized number PCR cycle number, sufficient to generate library yield for Illumina sequencing while avoiding unnecessary overamplification.ChIP-seq and [...]

What is the expected yield of DNA generated by the DNA SMART™ ChIP-Seq Kit?

The expected yield of DNA SMART is >5 ng/µl (>20–25 nM). Lower yield (2–3 ng/µl) may be expected for very low inputs (100 pg), but still enough for sequencing. If you obtain >25 ng/µl, consider reducing the number of cycles to minimize potential biases.ChIP-seq and small RNA-seq [...]

What is the cumulative size of adapters incorporated in the the final DMA SMART ChIP-Seq library?

Together, the DNA SMART Oligo, DNA SMART Poly(dA) Primer, and the Indexing Primers for Illumina® add 153 bp to the initial DNA fragment length.ChIP-seq and small RNA-seq [...]

Why is there no size selection and clean-up not before the PCR step within the DMA SMART ChIP-Seq protocol as in other kits?

he DNA SMART ChIP-Seq Kit does not use ligation, and high-quality ChIP-seq libraries can be obtained without Pre-PCR size selection. Based on our data that pre-PCR size selection reduces the complexity of the final library, with increased PCR duplicatesChIP-seq and small RNA-seq [...]

What size fragments can the DNA SMART ChIP-Seq Kit accommodate?

DNA fragments longer than 100 bp can be used with the DNA SMART ChIP-Seq Kit. If desired, fragments of several kb can easily be amplified; however, this kit has been tested specifically with ChIP DNA fragments ranging from 100 to 500 bp (with an average of 200–400 bp).ChIP-seq and small RNA-seq [...]