Why is there no size selection and clean-up not before the PCR step within the DMA SMART ChIP-Seq protocol as in other kits?
Why is there no size selection and clean-up not before the PCR step within the DMA SMART ChIP-Seq protocol as in other kits?
he DNA SMART ChIP-Seq Kit does not use ligation, and high-quality ChIP-seq libraries can be obtained without Pre-PCR size selection. Based on our data that pre-PCR size selection reduces the complexity of the final library, with increased PCR duplicates