Can the Guide-it SNP Screening Kit be used to detect successful homologous recombination (HR) in edited populations?

Although the Guide-it SNP Screening Kit was specifically intended for detection of SNPs in clonal cell lines, it can be used to detect successful HR in an edited population with a detection limit around 5%*. Download our poster to see data relating to this application. Contact our technical support if you need more information about how to use the kit for the detection of HR. *For this [...]

Can the Guide-it SNP Screening Kit be used for genotyping?

Yes, you will need to design one specific flap-probe per possible nucleotide at the target site and perform independent assays.Gene editing [...]

Can I use the kit if I have no idea what the substitution is?

Each assay performed with the Guide-it SNP Screening Kit is designed to detect a specific single-nucleotide substitution at a particular genomic location. The sequence of the flap-probe oligo determines which nucleotide will be detected at a chosen target site. To be able to identify any nucleotide at a given site, one must perform four independent assays, with each assay using a different [...]

Does the Guide-it SNP Screening Kit allow for detection of any nucleotide substitution regardless of the genomic target site?

Yes. The Guide-it SNP Screening Kit has been used successfully to analyze SNPs at 18 different loci within the human genome (corresponding to 14 different genes).Gene editing [...]

Can a qPCR thermal cycler be used for fluorescence detection step of Guide-it SNP Screening Kit?

Yes, a qPCR thermal cycler can be used. The Guide-it SNP Screening Kit employs an end-point assay that doesn't require real-time monitoring of the flapase reaction, but a qPCR machine can be used during the enzymatic assay to periodically measure fluorescence (e.g., every 3 minutes) to follow the kinetics of the reaction and upon completion of the reaction.Gene editing [...]