Can a qPCR thermal cycler be used for fluorescence detection step of Guide-it SNP Screening Kit?
Can a qPCR thermal cycler be used for fluorescence detection step of Guide-it SNP Screening Kit?
Yes, a qPCR thermal cycler can be used. The Guide-it SNP Screening Kit employs an end-point assay that doesn't require real-time monitoring of the flapase reaction, but a qPCR machine can be used during the enzymatic assay to periodically measure fluorescence (e.g., every 3 minutes) to follow the kinetics of the reaction and upon completion of the reaction.