What ThruPLEX kit do you recommend for NIPT (Non-invasive prenatal testing)? 

ThruPLEX Plasma-seq or ThruPLEX DNA-seq HV/Tag-seq HV kits can be used Non-invasive prenatal testing using cell-free DNA from liquid biopsies such as plasma, urine, serum, or any biological fluid. DNA-seq [...]

What is the recommended sequencing depth for detecting CNV?

PicoPLEX is the best technology for CNV (copy number variant) detection. The resolution of the CNV detection for PicoPLEX DNA-Seq is about 5 – 10 MB. At this level of binning, any aneuploidy in the genome will be detectable. The PicoPLEX GOLD DNA-seq kit has much better resolution (100 – 300 Kb) of CNV detection and much more uniform coverage.DNA-seq [...]

How does the smaller number of UMI (144) provide a complete diversity of tagged molecules in ThruPLEX Tag-seq kits?

First of all, there are no identical fragments with the exact same shearing pattern or same extremities at both 5’ and 3’ ends. So, we can say there is extremely low chance of having identical fragment with the same ends. Secondly, there is extremely low chance of ligation of the same UMI combination to both ends of a fragment even there were identical fragments. Thirdly, the diversity comes [...]

What is the difference between ThruPLEX Tag-seq and ThruPLEX Tag-seq HV kits?

The ThruPLEX Tag-seq kits are now considered as legacy kits that are supplied with the TruSeq CD adapters containing the Unique Molecular Tags (UMT). Each UMT is made of a stretch of six degenerate bases, providing more than 4,000 unique sequence combinations. Together, the UMTs on both sides of the fragment produce up to 16 million tags thus ensuring unique labeling of all fragments for [...]

What is the difference between ThruPLEX and ThruPLEX HV kits?

The main improvements of the HV kit are - *Larger input volume: 30ul (Older kits - 10 ul) *Increase input range: 200 ng (Older kits - 50 ng) This is a great improvement of the kit because cell-free DNA is often highly diluted and requires a higher input. Another change was the protocol optimizations: The HV kit protocol has Two PCR steps unlike the older versions which included [...]