Which kit should I choose for ChIP-seq – ThruPLEX DNA-seq or DNA SMART ChIP-seq kit?

Both kits are perfectly suitable for ChIP-seq applications. However, the major deciding factor should be if ChIP DNA single- or double-stranded. The ThruPLEX kit only works with double-stranded DNA of size between 50bp to 1000bp with an amount of 50pg to 200ng, while DNA SMART ChIP-seq kit is suitable for both – single and double stranded DNA of size between 200-400bp with amount of 100pg-10ng. [...]

Is it recommended to spike PhiX into a ThruPLEX DNA-Seq library prior to loading onto a flow cell?

Follow the manufacturer's instructions for using PhiX. For low-diversity libraries or if one is experiencing sequencing issues, the PhiX concentration may need to be increased. Please refer to the ThruPLEX DNA-Seq Kit User Manual for additional sequencing recommendations.DNA-seq [...]

Are there any special steps that need to be followed when loading a ThruPLEX DNA-Seq library onto a flow cell?

No, there are no special steps. ThruPLEX DNA-Seq libraries should be handled and processed in the same way as libraries generated using Illumina library preparation kits, such as the TruSeq® Nano DNA HT Sample Prep Kit.DNA-seq [...]

What concentration of ThruPLEX DNA-Seq library should be loaded onto a flow cell?

Please follow Illumina’s recommendations for the optimal loading concentration specific to the version of flow cell you are using. For sequencing on the Illumina MiSeq®, v3, we suggest that you load 14–15 pM of ThruPLEX DNA-Seq library.DNA-seq [...]

Which Illumina NGS systems can I use to sequence the libraries prepared using the ThruPLEX DNA-Seq Kit?

Libraries prepared with the ThruPLEX DNA-Seq Kit are compatible with all Illumina sequencing platforms, including the HiSeq®, MiSeq®, NextSeq® 500, GAIIx™, NovaSeq™, and MiniSeq™.DNA-seq [...]