What rRNAs are removed by ZapR/ R-probes?

R-probes target mammalian rRNA (18S and 28S) and human mitochondrial rRNA (m12S and m16S) sequences only. R-probes do not target 5S and 5.8S rRNAs or the rRNA precursors. non-human mitochondrial rRNA.Stranded RNA-seq for mammalian [...]

What is the mechanism of the removal of rRNA sequences within the workflow of the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

A proprietary ZapR technology in conjunction with R-Probes, selectively removes mammalian rRNA (18S and 28S) and human mitochondrial rRNA (m12S and m16S) sequences from pre-amplified double-stranded cDNA.Stranded RNA-seq for mammalian [...]

Do I have to remove rRNA prior to the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian workflow?

No, the removal of rRNA sequences from pre-amplified double-stranded cDNA is incorporated into the workflow of the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian shown in the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian User Manual - Figure 2. Schematic of technology in the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian.Stranded RNA-seq for mammalian [...]

Can I use 100pg total RNA as input into the v3 Pico workflow?

Lower than recommended RNA input quantities used for the v3 Pico workflow may generate higher duplicate rates due to low sample complexity.Stranded RNA-seq for mammalian [...]

Can I use more than 10ng total RNA as input into the v3 Pico workflow?

Using higher than recommended RNA input quantities into the v3 Pico workflow are not recommended, since it may contribute to a higher % of rRNA sequences in the final v3 Pico library.Stranded RNA-seq for mammalian [...]