Do I have to remove rRNA prior to the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian workflow?

Do I have to remove rRNA prior to the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian workflow?

No, the removal of rRNA sequences from pre-amplified double-stranded cDNA is incorporated into the workflow of the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian shown in the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian User Manual - Figure 2. Schematic of technology in the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian.

Stranded RNA-seq for mammalian samples

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