What are the input requirements for the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian, utilizing a random priming for the first-strand cDNA synthesis is compatible with 250pg-10ng total mammalian RNA of various RINs or 10-1,000 whole intact cellsSMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian, utilizing a random priming for the first-strand cDNA synthesis is compatible with 250pg-10ng total mammalian RNA [...]

What is the mechanism of sequencing library construction using the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

The workflow of the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian compatible with 250pg-10ng total mammalian RNA of various RINs or 10-1,000 whole intact cells, utilizes a random priming for the first-strand cDNA synthesis and incorporates the removal of rRNA sequences, generating Illumina-ready library representing coding and non-coding RNAs with strand information as shown in [...]

What is the duplicate rate in the final SMARTer Stranded v2 - Pico cDNA library?

Duplicate rate depends on the input cell/RNA complexity and the sequencing depth. Low quantity RNA/ cell inputs have lower complexity resulting in higher duplicate rates in the final cDNA libraries. Compromised RNA may generate higher duplicate rate. High sequencing depth will generate a higher duplicate rate, especially for low complexity samples.Stranded RNA-seq for mammalian [...]

How does SMARTer Stranded v2 - Pico cDNA sequencing metrics correlate between RNA and cell inputs?

SMARTer Stranded v2 - Pico sequencing metrics shows a good correlation between cells and purified RNA inputs.Stranded RNA-seq for mammalian [...]

What is the expected gene body coverage for the SMARTer Stranded v2 - Pico library?

There is a slight 5’-end bias in the SMARTer Stranded v2 - Pico cDNA, which is common for randomly primed libraries.Stranded RNA-seq for mammalian [...]