What is the mechanism of sequencing library construction using the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?
What is the mechanism of sequencing library construction using the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?
The workflow of the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian compatible with 250pg-10ng total mammalian RNA of various RINs or 10-1,000 whole intact cells, utilizes a random priming for the first-strand cDNA synthesis and incorporates the removal of rRNA sequences, generating Illumina-ready library representing coding and non-coding RNAs with strand information as shown in the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian User Manual - Figure 2. Schematic of technology in the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian