Can I use R-Probes v2/ ZapR v2 for the removal of rRNA sequences in other cDNA synthesis workflows or with pre-synthesized double-stranded cDNA?

No, R-Probes v2/ ZapR v2 removal of rRNA sequences has been designed to work exclusively as an integral part of the workflows of the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian.Stranded RNA-seq for mammalian [...]

What rRNA species are not removed by the ZapR v2?

R-Probes v2/ ZapR v2 do not remove 5S, 5.8S rRNAs, rRNA precursors, mt rRNAs from mammalian species other than humans.Stranded RNA-seq for mammalian [...]

What is the mechanism of the removal of rRNA sequences within the workflow of the SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian?

A proprietary ZapR technology in conjunction with R-Probes, selectively removes mammalian rRNA (18S and 28S) and human mitochondrial rRNA (m12S and m16S) sequences from pre-amplified double-stranded cDNA.Stranded RNA-seq for mammalian [...]

What are the input requirements for the SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian?

SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, utilizing a random priming for the first-strand cDNA synthesis is compatible with 250pg-10ng total mammalian RNA of various RINs or 50-1,000 whole intact cells.Stranded RNA-seq for mammalian [...]

What is the mechanism of sequencing library construction using the SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian?

SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian compatible with 250pg-10ng total mammalian RNA of various RINs or 50-1,000 whole non-damaged cells, utilizes a random priming for the first-strand cDNA synthesis, incorporates the removal of rRNA sequences, generating Illumina-ready library representing coding and non-coding RNAs with strand information.Stranded RNA-seq for mammalian [...]