What is the mechanism of sequencing library construction using the SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian?
What is the mechanism of sequencing library construction using the SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian?
SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian compatible with 250pg-10ng total mammalian RNA of various RINs or 50-1,000 whole non-damaged cells, utilizes a random priming for the first-strand cDNA synthesis, incorporates the removal of rRNA sequences, generating Illumina-ready library representing coding and non-coding RNAs with strand information.