Is there a way to improve transduction efficiency?

Below are some tips on how to improve transduction efficiency using RetroNectin reagent. 1. We recommend preparing retrovirus supernatant at a high titer (>106 cfu/ml is desirable). To achieve transduction efficiencies of 60–70%, concentrate the retrovirus solution (e.g., measured titer = 1.15 x 106) more than 10-fold and use it for pre-loading. 2. Pre-load viral particles onto RetroNectin [...]

Can cell-culture bags (e.g., X-fold bags) be coated with RetroNectin reagent?

Yes, X-fold bags can be coated with RetroNectin reagent. Below is a protocol that has been tested for an X-fold Bag (85 cm2): 1. Coat the bag with RetroNectin reagent* overnight at 4°C, or for 2 hours at room temperature. *Use at least 9 ml of RetroNectin solution (20 µg/ml) per bag. 2. Wash with PBS (30 ml x 3 times). 3. Add Retrovirus (4–6 hours). 4. Wash with PBS (30 ml). 5. Add cells (1 x [...]

Does Takara Bio provide a clinical-grade RetroNectin reagent?

Yes. RetroNectin GMP grade (Cat. # T202) is manufactured as a quality-assured product according to guidelines for Good Manufacturing Practice (GMP) for Investigational Products and can be used for ex vivo clinical applications (limited to investigational use only).RetroNectin [...]

Does RetroNectin reagent have to be filter sterilized?

RetroNectin reagent is provided as a sterile solution. Therefore, filtration is not necessary.RetroNectin [...]

For how long are pre-coated RetroNectin plates stable?

Pre-coated RetroNectin plates can be stored at 4°C and are stable for 1–2 months (although we recommend using them as soon as possible). Do not store pre-coated plates at –20°C.RetroNectin [...]