Can RetroNectin reagent be coated on plates that are also coated with collagen?

If the use of double-coated plates is necessary, please test the following two coating methods: -coat with RetroNectin reagent first, then collagen -coat with collagen first, then RetroNectin reagent If transfection efficiencies are very low, perform the transduction procedure using a RetroNectin-coated non-tissue culture treated plate as described in the user manual. After infection, incubate [...]

What type of tissue culture plate can be coated with RetroNectin reagent?

Non-coated tissue culture plates or dishes should be used. Takara Bio R&D scientists have tested both coated and non-coated plates and found that RetroNectin coating efficiency is reduced by 1/5 for coated plates. Experimental data indicate that transduction efficiency is above 30% when using RetroNectin-coated, non-tissue culture treated plates, but only 1–3% when using RetroNectin-coated [...]

Can RetroNectin reagent be used in combination with Polybrene?

Do not use both reagents simultaneously. Concomitant use of RetroNectin reagent and Polybrene will decrease gene-transfer efficiency.RetroNectin [...]

How much cytotoxicity is typically observed when using RetroNectin reagent?

Less cytotoxicity has been observed with RetroNectin reagent compared to Polybrene. For example, there is almost no effect on cellular growth when NIH 3T3 cells are grown in medium containing 0.25 mg/ml of RetroNectin reagent.RetroNectin [...]

Does RetroNectin reagent work for retroviruses packaged with different envelopes?

RetroNectin reagent can be used to enhance transduction of VSV-g, ecotropic, and amphotropic retroviruses and lentiviruses. In addition, this product is compatible with retroviruses with a GALV-type envelope.RetroNectin [...]