What kit is recommended for whole cells or ultra-low input total RNA samples: SMARTer Ultra low kits (dT-primed)

SMARTer Ultra low kits (including the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing) generate cDNA from 1–1,000 intact cells or 10 pg–10 ng total RNA samples. Since the SMARTer Ultra low and SMART-Seq v4 kits use oligo(dT) priming for first-strand cDNA synthesis, total RNA samples must be of high quality, with an RNA integrity number (RIN) ?8 to ensure the availability of full-length mRNA [...]

What kit is recommended for whole single cells: SMART-Seq Single Cell Kit (full-length mRNA-seq)

Created specifically for single-cell and nuclei applications—and especially for cells known to have low RNA content, such as PBMCs—the SMART-Seq Single Cell Kit clearly outperforms previously published protocols (such as Smart-seq2) and existing commercial kits in terms of sensitivity and reproducibility. The improved full-length chemistry within this validated kit is associated with a higher [...]

Which kit is right for my application?

Illumina indexes and adapters are integrated during cDNA amplification with the SMARTer Stranded RNA-Seq Kit (including the HT version) and the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian. No additional library preparation is needed. (see ""Which indexes are included in SMARTer stranded kits?""). Not all indexes can be pooled together, consult the Illumina literature (such as the [...]

Can the Cogent NGS Immune Profiler Software analyze only Read1 sequencing data?

No. Read 2 is essential for the Cogent NGS Immune Profiler Software analysis, since it carries UMIs as shown in the SMARTer® Human BCR IgG IgM H/K/L Profiling Kit User Manual - Figure 5. SMARTer Human BCR IgG IgM H/K/L Profiling library structure.Immune profiling [...]

What is the source of the Control Total RNA included in the SMARTer® Human BCR IgG IgM H/K/L Profiling Kit?

Control Total RNA, included in the SMARTer® Human BCR IgG IgM H/K/L Profiling Kit is Human Spleen Total RNA.Immune profiling [...]