What kit is recommended for whole single cells: SMART-Seq Single Cell Kit (full-length mRNA-seq)
What kit is recommended for whole single cells: SMART-Seq Single Cell Kit (full-length mRNA-seq)
Created specifically for single-cell and nuclei applications—and especially for cells known to have low RNA content, such as PBMCs—the SMART-Seq Single Cell Kit clearly outperforms previously published protocols (such as Smart-seq2) and existing commercial kits in terms of sensitivity and reproducibility. The improved full-length chemistry within this validated kit is associated with a higher number of useful reads and more genes detected than other popular full-length methods. The kit's plate-based workflow accepts single cells from FACS or other methods as input and uses an oligo(dT)-priming approach for first-strand cDNA synthesis.
SMART-Seq full-length chemistry has reached a new level. Read our technical note to see how you can discover more from your scRNA-seq experiments.