What kit is recommended for whole cells or ultra-low input total RNA samples: SMARTer Ultra low kits (dT-primed)
What kit is recommended for whole cells or ultra-low input total RNA samples: SMARTer Ultra low kits (dT-primed)
SMARTer Ultra low kits (including the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing) generate cDNA from 1–1,000 intact cells or 10 pg–10 ng total RNA samples. Since the SMARTer Ultra low and SMART-Seq v4 kits use oligo(dT) priming for first-strand cDNA synthesis, total RNA samples must be of high quality, with an RNA integrity number (RIN) ?8 to ensure the availability of full-length mRNA templates required for cDNA synthesis. Ribosomal RNA (rRNA) removal or DNase treatment of RNA samples is not required for these kits. These kits selectively and efficiently amplify polyA+ RNA regardless of the presence of rRNA or genomic DNA.There are several Takara Bio kits for single-cell RNA-seq that have superior performance, including the SMARTer Ultra low RNA kit for the Fluidigm C1 System for higher-throughput experiments.