Is the SMARTer Human TCR a/b Profiling Kit v2 suitable for TCR profiling of single cells?

No, SMARTer Human TCR a/b Profiling Kit v2 requires at least 1,000 purified T cells as input." Does SMARTer® Human TCR a/b Profiling Kit allow alpha-beta chain pairing? "No, alpha-beta chain pairing is possible at a single cell level and requires also cell barcoding: if alpha and beta chains carry the same cell barcode, they are from the same cell. Alpha-beta chain pairing is possible using [...]

Does SMARTer Human TCR a/b Profiling Kit v2 require rRNA depletion prior to cDNA synthesis?

No, SMARTer Human TCR a/b Profiling Kit v2 utilizes oligo(dT) priming for the first-strand cDNA synthesis (annealing to poly(A) mRNAs) followed by TCR-specific amplification, thus generating TCR-specific cDNA. It is advisable to use high integrity (RIN>7) input RNA for the SMARTer® Human TCR a/b Profiling KitImmune profiling [...]

What are the input requirements for the SMARTer Human TCR a/b Profiling Kit v2?

SMARTer Human TCR a/b Profiling Kit v2 utilizing oligo(dT) priming for the first-strand cDNA synthesis is compatible with high integrity total RNA (RIN>7) using 10ng-1ug obtained from peripheral blood leukocytes, 20 ng to 200 ng of total RNA obtained from whole blood, 1 ng to 100 ng of total RNA obtained from T cells, or with 1,000 to 10,000 purified, whole T cells.Immune profiling [...]

What is the size of the adapters added to the SMARTer® Human TCR-v2 a/b library?

Total size of adapter-dimer in the TCR-v2 library is about 159bp including UMI, UMI-linker ,3 GC-rich nucleotides of the TCR SMART UMI Template Switching Oligo - as shown in the SMARTer Human TCR a/b Profiling Kit v2 User Manual - Figure 4. SMARTer human TCR profiling v2 library structure.Immune profiling [...]

What brand of beads is compatible with theSMARTer Human TCR a/b Profiling Kit v2 workflow?

NucleoMag NGS Clean-up and Size Select (available from Takara Bio; 5-ml size: Cat. No. 744970.5; 50-ml size: Cat. No. 744970.50; 500-ml size: Cat. No. 744970.500) or AMPure XP PCR purification kit (Beckman Coulter; 5-ml size: Cat. No. A63880; 60-ml size: Cat. No. A63881) is an appropriate substitute, using the same bead:sample ratios as recommended for the NucleoMag NGS Clean-up and Size [...]