Does SMARTer Human TCR a/b Profiling Kit v2 require rRNA depletion prior to cDNA synthesis?
Does SMARTer Human TCR a/b Profiling Kit v2 require rRNA depletion prior to cDNA synthesis?
No, SMARTer Human TCR a/b Profiling Kit v2 utilizes oligo(dT) priming for the first-strand cDNA synthesis (annealing to poly(A) mRNAs) followed by TCR-specific amplification, thus generating TCR-specific cDNA. It is advisable to use high integrity (RIN>7) input RNA for the SMARTer® Human TCR a/b Profiling Kit