Is the SMARTer Human TCR a/b Profiling Kit v2 suitable for TCR profiling of single cells?
Is the SMARTer Human TCR a/b Profiling Kit v2 suitable for TCR profiling of single cells?
No, SMARTer Human TCR a/b Profiling Kit v2 requires at least 1,000 purified T cells as input."
Does SMARTer® Human TCR a/b Profiling Kit allow alpha-beta chain pairing? "No, alpha-beta chain pairing is possible at a single cell level and requires also cell barcoding: if alpha and beta chains carry the same cell barcode, they are from the same cell. Alpha-beta chain pairing is possible using SMARTer® Human scTCR a/b Profiling Kit, utilizing cell barcoding.