How many hiPS cell-derived hepatocytes can I expect to get from one kit?

Approximately 5 x 106 hepatocytes, which corresponds to 50 cm2 of confluent hepatocytes, e.g., 150 wells in 96-well plates or 24 wells in a 24-well plate. Stem Cell [...]

What features can I expect from the hepatocytes derived with the Cellartis iPS Cell to Hepatocyte Differentiation System?

Cellartis enhanced hiPS-HEP cells are produced using the same protocol as in the differentiation kits. Therefore, one can expect the same features as seen for Cellartis enhanced hiPS-HEP cells, with the expected cell line variability regarding, e.g., CYP activities. See our Tech Notes for more information. Stem Cell [...]

On what day can I start using the hepatocytes?

When using the Cellartis iPS Cell to Hepatocyte Differentiation System, we consider cells to be hepatocytes after about 21 days of culture. However, the hepatocytes continue to mature after Day 21. For example, fetal marker AFP is gradually downregulated between Days 21–39. The hepatocytes can thus be used from Day 21 (counting from the start of differentiation from hiPS cells, corresponding to [...]

How do the hepatocytes derived with this system compare to human primary hepatocytes?

One of the major differences is that the functionality of primary hepatocytes declines rapidly in culture due to a dedifferentiation process, whereas hepatocytes derived with our system can be kept in culture for several weeks with maintained functionality. In addition, different batches of human primary hepatocytes show large donor variations due to the pronounced metabolic diversity in the [...]

What happens to the hPS cell-derived hepatocytes after Day 32? Do they lose activity, no longer look like hepatocytes, or die?

The hepatocytes can be maintained for 10 additional days, to Day 42, using Cellartis Hepatocyte Maintenance Medium (sold separately as Cat. No. Y30051). After Day 42, the hepatocytes may lose activity, detach from the culture vessel, and/or start to die. Stem Cell [...]