On what day can I start using the hepatocytes?
On what day can I start using the hepatocytes?
When using the Cellartis iPS Cell to Hepatocyte Differentiation System, we consider cells to be hepatocytes after about 21 days of culture. However, the hepatocytes continue to mature after Day 21. For example, fetal marker AFP is gradually downregulated between Days 21–39. The hepatocytes can thus be used from Day 21 (counting from the start of differentiation from hiPS cells, corresponding to Day 14 after the start of differentiation from DE cells), but the optimal day may vary depending on your applications and maturity requirements.