I observe dome-like structures in Cellartis enhanced hiPS-HEP cell culture. Is this normal?

Occasionally, these domes appear, but they do not affect overall cell performance. In these structures, the hepatocytes have detached from the coating, possibly because they were growing very densely. To prevent cell loss, please be careful when changing the media; always leave 10% of the media in each well.Stem Cell [...]

I observe droplets in Cellartis enhanced hiPS-HEP cell culture. Is this normal?

These liquid extrusions are normal. In time-lapse recordings, we have observed that hepatocytes release these drops into the media and remain healthy afterward. This is not a sign of cell death, and cultures with these drops perform normally in activity assays. We have stained the cultures with Oil Red O, and these drops were not stained, indicating that they are not lipid droplets. Although the [...]

How were CYP enzyme activities measured in Cellartis enhanced hiPS-HEP cells

Liquid chromatography/mass spectrometry (LC/MS) was used to measure the formation of these specific metabolites: acetaminophen (CYP1A), 4-OH-Diclofenac (CYP2C9), OH-Bufuralol (CYP2D6), and 1-OH-Midazolam (CYP3A). The CYP activities of Cellartis Enhanced hiPS-HEP cells were analyzed at Days 4, 12, and 19 after thawing as part of routine quality control. LC/MS analysis was performed at [...]

Is it possible to proliferate Cellartis enhanced hiPS-HEP cells?

No, Cellartis enhanced hiPS-HEP cells are terminally differentiated hepatocytes that are unable to proliferate. Stem Cell [...]

Can Cellartis enhanced hiPS-HEP cells go without changing media over the weekend?

Yes, but add 50% more media on Friday (i.e., 1.5 ml/well of a 24-well plate and 230 µl/well of a 96-well plate). It is best to change the media late on Friday and early on Monday. Stem Cell [...]