I observe droplets in Cellartis enhanced hiPS-HEP cell culture. Is this normal?
I observe droplets in Cellartis enhanced hiPS-HEP cell culture. Is this normal?
These liquid extrusions are normal. In time-lapse recordings, we have observed that hepatocytes release these drops into the media and remain healthy afterward. This is not a sign of cell death, and cultures with these drops perform normally in activity assays. We have stained the cultures with Oil Red O, and these drops were not stained, indicating that they are not lipid droplets. Although the exact nature of the drops is unknown, cell performance remains intact despite their presence.
