Are Cellartis enhanced hiPS-HEP cells available from several hiPS cell lines?

Yes, Cellartis enhanced hiPS-HEP cells are available from three different cell lines: ChiPSC12, ChiPSC18, and ChiPSC22. Hepatocytes derived from different hiPS cell lines model the genetic variation between individuals, therefore, the cells display phenotypic variation. Stem Cell [...]

Are Cellartis enhanced hiPS-HEP cells genetically engineered?

No. In some hepatocytes genetic engineering is used to improve hepatic function. Instead, we developed a culture protocol that substantially improves functionality. Each hiPS cell line—the source of the Cellartis hepatocytes—was reprogrammed using an integrative, retroviral technique. We tested for reverse transcriptase activity in the hiPS cell lines to confirm the absence of [...]

What is the source of Cellartis enhanced hiPS-HEP cells?

Cellartis enhanced hiPS-HEP cells are differentiated from human induced pluripotent stem (hiPS) cell lines ChiPSC12, ChiPSC18, and ChiPSC22. These lines were created by reprogramming human dermal fibroblasts with defective polycistronic retrovirus technology, which includes a retroviral vector containing Oct4, Sox2, Klf4, and c-Myc (pRV-OCT4-SOX2-KLF4-MYC; the vector map is proprietary). Reverse [...]

Do you need Licensing to use Takara Tet-On 3G systems?

Use of tetracycline-controllable expression systems (the ""Tet Technology"") is covered by a series of patents which are proprietary to TET Systems GmbH & Co. KG. There are specific licensing procedures that apply to for-profit companies and institutions. To determine whether you need a license to place an order, please log in to the Takara Bio website or contact the Takara Bio licensing [...]

How to detect Tet-On and Tet-Off proteins with the TetR Monoclonal Antibody

Detect Tet-On and Tet-Off proteins with the TetR Monoclonal Antibody The TetR Monoclonal Antibody (Clone 9G9) was raised against an essential component of the Tet-On and Tet-Off transactivator proteins. Use the TetR Monoclonal Antibody to simplify the process of developing a Tet-inducible expression system: detect Tet-On 3G, Tet-On Advanced, and Tet-Off Advanced proteins on Western blots.Read [...]