Do I need to treat DNA with RNase prior to DNA SMART library preparation?

No. Residual RNases in the sample may compromise the success of library preparation. Furthermore, the Terminal Deoxynucleotidyl Transferase will not use RNA as a template in the T-tailing step, and the use of a poly(dA) primer, instead of a poly(dT) primer, makes priming to RNA unlikely.ChIP-seq and small RNA-seq [...]

Did you compare sequencing metrics of DNA recovered from ChIP assay using ChIP Elute Kit versus using traditional methods of DNA recovery from ChIP assay

ChIP-seq libraries generated from DNA processed by the ChIP Elute Kit are similar to those processed by traditional methods of cross-linking reversal and elution, as shown in the Technical Note - Ligation-free ChIP-seq library preparation - Sequencing metrics comparing ChIP-seq libraries generated with ChIP Elute Kit or traditional cross-linking reversal methodsChIP-seq and small RNA-seq [...]

How does the ChIP Elute work?

The ChIP Elute Kit, compatible with any upstream ChIP protocol, allows for simultaneous elution and crosslinking reversal of nuclear protein-DNA complexes in a single step, generating single-stranded DNA which is purified and concentrated using ChIP-grade reagents included in the kit. The resulting single-stranded DNA (ssDNA) is compatible with qPCR applications or direct sequencing library [...]

What kit do you recommend for DNA recovery from the ChIP assay for compatibility with the DNA SMART™ ChIP-Seq Kit?

We recommend using the ChIP Elute Kit (Cat. No. 634887) allowing for DNA elution from Protein A/G agarose or magnetic beads and cross-linking reversal in a single step in about 1 hr. The resulting DNA is single stranded (ssDNA) which is purified and concentrated with the ChIP-grade reagents provided in the kit.ChIP-seq and small RNA-seq [...]

What is the size distribution of Control Fragmented Human gDNA, included in the DNA SMART ChIP-Seq kit?

Control Fragmented Human gDNA includes fragments ranging from 75 bp to 900 bp.ChIP-seq and small RNA-seq [...]