Do I need to treat DNA with RNase prior to DNA SMART library preparation?
No. Residual RNases in the sample may compromise the success of library preparation. Furthermore, the Terminal Deoxynucleotidyl Transferase will not use RNA as a template in the T-tailing step, and the use of a poly(dA) primer, instead of a poly(dT) primer, makes priming to RNA unlikely.
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