Takara enzyme works with PCR-inhibiting agents

TaKaRa Ex Taq DNA Polymerase is exceptionally robust, even in the presence of PCR inhibitors such as polyphenols found in crude DNA extracts from plant tissue. PrimeSTAR GXL DNA Polymerase is recommended when high fidelity is also required. Although PrimeSTAR GXL polymerase generally produces satisfactory results with the standard protocol, doubling the enzyme concentration may improve results [...]

Takara enzyme for bisulfite-treated DNA

Several choices are available: 1. TaKaRa EpiTaq HS (for bisulfite-treated DNA) is optimized for PCR amplification using bisulfite-treated DNA templates that contain uracil. This enzyme includes a hot-start antibody and is designed for use during methylation analysis, including COBRA and bisulfite-sequencing analyses. 2. The EpiScope MSP Kit is designed specifically for methylation-specific PCR [...]

Takara enzyme for AT-rich target sequences

TaKaRa Ex Taq DNA Polymerase and PrimeSTAR GXL DNA Polymerase are effective for amplifying AT-rich target sequences, such as genomic DNA containing introns or AT-rich mitochondrial DNA. We recommend PrimeSTAR GXL DNA Polymerase for amplifying AT-rich templates with high accuracy. In contrast to other PCR enzymes, PrimeSTAR GXL polymerase can amplify targets containing >60% AT content using a [...]

Takara enzyme for Fast PCR

We have several PCR enzymes that can be used for fast reactions. 1. Speed and fidelity—PrimeSTAR Max DNA Polymerase contains a proprietary elongation factor and exhibits excellent priming efficiency, allowing extension times as short as 5 sec/kb and an annealing time of only 5 sec. This enzyme is recommended for cloning and expression studies. 2. High-throughput applications and fast colony [...]

Takara enzyme for Long-range PCR

PrimeSTAR GXL DNA Polymerase is recommended when both length (>6 kb) and fidelity are factors. Amplification of 30-kb products from human genomic DNA templates has been accomplished with this enzyme. Takara LA Taq DNA polymerase can also be used for long-range PCR; this enzyme is recommended when length and robust amplification are priorities.PCR [...]